Application | Comment | Organism |
---|---|---|
nutrition | possible usage as feed additive for animals in order to diminish health problems and enhance proliferation of beneficial microflora | Pseudobutyrivibrio xylanivorans |
Cloned (Comment) | Organism |
---|---|
partial DNA and amino acid sequencing | Pseudobutyrivibrio xylanivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
separation of 2 isozymes with different MW by native PAGE | Pseudobutyrivibrio xylanivorans |
30000 | - |
x * 30000, SDS-PAGE | Pseudobutyrivibrio xylanivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudobutyrivibrio xylanivorans | - |
rumen bacteria, strain Mz5T, DSM14809, enzyme XynT, 2 isozymes | - |
Purification (Comment) | Organism |
---|---|
by ammonium sulfate precipitation and hydrophobic interaction chromatography, to homogeneity, 2 isozymes | Pseudobutyrivibrio xylanivorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.5 | - |
purified enzyme | Pseudobutyrivibrio xylanivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity against other polysaccharides, no arabinofuranosidase and beta-xylosidase activity, enzyme contains no xylan-binding domain | Pseudobutyrivibrio xylanivorans | ? | - |
? | |
xylan + H2O | substrate from brichwood, beechwood, or oat spelt soluble fraction, the latter being the best substrate, endoxylanase activity | Pseudobutyrivibrio xylanivorans | xylooligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Pseudobutyrivibrio xylanivorans |
Synonyms | Comment | Organism |
---|---|---|
endoxylanase | - |
Pseudobutyrivibrio xylanivorans |
More | enzyme belongs to family 11 of glycosyl hydrolases | Pseudobutyrivibrio xylanivorans |
XynT | - |
Pseudobutyrivibrio xylanivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
- |
Pseudobutyrivibrio xylanivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
- |
Pseudobutyrivibrio xylanivorans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudobutyrivibrio xylanivorans | purified isozymes with 2 pI values, isoelectric focusing | - |
4.9 |
Pseudobutyrivibrio xylanivorans | purified isozyme with 2 pI values, isoelectric focusing | - |
6 |